WORKSHOPS

We are pleased to offer five exciting workshops that will be held Saturday, Nov. 10 and Sunday, Nov. 11 2018, from 8:30am-4:30pm each day.  Each workshop has a total limit of participants due to the practical nature and equipment needs of each workshop.  Please read the descriptions carefully before making your selections.  There is a $40.00 US workshop fee for each workshop and lunch is included both days.  Once workshops fill, the workshop leads will contact you in more detail about workshop objectives and shipping samples if needed.  Please refer any workshop-related questions to the workshop leads.

I. Workshop on Genetic Tools and Live Imaging in Cephalopods

  • Workshop lead(s): Eric Edsinger
  • email: eedsinger@mbl.edu
  • Total # participants: 20
  • Pre-preparation by participants:  Yes

This 2-day workshop will cover 1) identifying genes of interest in annotated genomes (discussion and hands-on with computer software), 2) designing transgenic reporters, biosensors, and CRISPR-Cas9 guide RNAs (discussion and hands-on with computer software), 3) injection of mRNA/constructs/CRISPR-Cas9 in one or more cephalopod species (hands-on injection of pygmy squid and/or other species), 4) live imaging approaches for light-based genetic tools (discussion and hands-on live imaging of fluorescent dyes, proteins, and/or biosensors), and 5) applications of genomic resources and genetic tools to cephalopod biology, emerging genetic models, and other future prospects (discussions).  The workshop will produce a number of embryos and hatchlings expressing injected mRNAs, transgenic constructs, or genome edited genes which could possibly lead to a co-authored publication.

II. Paralarval and juvenile cephalopods: an updated identification guide

  • Workshop lead(s): Erica Vidal, Liz Shea and Heather Judkins 
  • email: ericavidal2000@yahoo.com.br
  • Total # participants:  25
  • Pre-preparation/ possibly shipping/bringing samples to St. Peteresburg:  Yes

The “Larval” and juvenile cephalopods: A manual for their identification, was published in 1992 and is outdated, as several recent publications have broaden our knowledge of the taxonomic status of cephalopods early life stages. The provisional identification key provided in that manual has several problems and badly needs a revision.  Thus, the goal of this workshop is to compile and synthesize existing taxonomic information to create an update identification guide of cephalopods early life stages. Currently, the identification of early stages of many species is problematic and any improvement in this regard will promote a better understanding of the life cycles and population dynamics of these species. This workshop also would be an excellent opportunity to disseminate knowledge on the identification of cephalopods early life stages among the participants with a “hands on” approach.   The expected output form this workshop is creating a publication of an identification guide for paralarval and juvenile cephalopods

III. Hard structures of cephalopods and their application in your field of study

  • Workshop lead(s):  Alexander I. Arkhipkin, Catalina Perales-Raya and Fedor Lischenko 
  • Email: fedor-ln@yandex.ru
  • Total # participants: 25
  • Pre-preparation and/or shipping/bringing samples:  Yes

For several decades, hard structures of cephalopod mollusks have been used as practical and reliable tools in a wide variety of studies. Nowadays the age, growth and maturity rates could be estimated using increments on statoliths, beaks or vestigial shells, taxonomic or even population affiliation of individual could be determined by the shape of hard structures. Moreover, hard structures alone could provide enough data to describe the animal's life cycle. At the moment the amount of information about species biology which could be obtained using hard structures, is literally staggering. It is easy to get confused in such a variety of methods and approaches. To solve this problem, we are going to discuss at this working group the modern challenges of cephalopod’s hard structures use and the latest achievements in this sphere. In addition to that, hands-on course of hard structure application for ageing and taxonomic studies is planned.  Expected product from discussion part of workshop is the article on modern methods of hard structures use, expected product form the second part of workshop - practical skills of age estimation and shape analysis obtained by participants.

IV. The biogeochemical role of cephalopods in the world’s oceans

  • Workshop lead(s): Henk-Jan Hoving
  • email: hhoving@geomar.de
  • Total # participants:  30-40
  • Pre-preparation by participants needed:  Yes

Cephalopods perform extensive vertical and horizontal migrations, they are key organisms in the oceanic foodweb as predators and prey, they have high growth and metabolic rates and they typically are monocyclic which in combination with mating and spawning aggregations may result in mass mortality events. All these aspects of cephalopod behavior and life history suggest that they are significant components of the energy flow and biogeochemical cycle of the world oceans. This workshop aims to bring scientists together who have an interest in the role of cephalopods in ocean biogeochemistry. During this workshop we will discuss several topics related to energy transfer and biogeochemistry (e.g. migration, consumption, respiration and excretion, terminal spawning), and participants are invited to give a short presentation, followed by a discussion. This will form the basis for a peer reviewed review article on the biogeochemical role of cephalopods

V. Cephalopod Science: the direction of future research and the relevance of new policies 

  • Workshop lead(s): Giovanna Ponte, Ian Gleadall and Graziano Fiorito
  • email: graziano.fiorito@szn.it
  • Expected # participants: 30-40
  • Pre-preparation needed by participants:  Yes (some readings prior to workshop)

 (1) A brainstorming session to identify likely avenues for novel ground-breaking research and their potential effects on and benefits to human society. 

(2) A discussion on the changes in policy for, e.g. experimentation on (and fisheries of) cephalopods occurring in different regions of the world and the potential effects of these changes on cephalopod research in both global and local contexts. 

(3) Preparations for a white-paper to summarize and report on (1) and (2).